Anti-Pan-Ras (Ab-3) Mouse mAb (RAS 10)

Analysis Note

Positive ControlSW480 or Y1 cells or breast carcinoma tissue

Application

Flow Cytometry (2.5 g/ml)Frozen Sections (2.5 g/ml)Immunoblotting (0.1-3 g/ml, chemiluminescent and colorimetric, see application references)Immunofluorescence (2.5 g/ml, see application references)Immunoprecipitation (1 g/sample)Paraffin Sections (2.5 g/ml, saponin pre-treatment required)

General description

Recognizes the ~21 kDa H-, K-, and N-Ras proteins in SW480 and Y1 cells and breast carcinoma tissue.

This Anti-Pan-Ras (Ab-3) Mouse mAb (RAS 10) is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of Pan-Ras (Ab-3).

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 myeloma cells (see application references). Recognizes the ~21 kDa Ras protein.

Immunogen

recombinant human p21 Ras

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Rodenhuis, S. et al., 1992. Cancer Res. (Suppl)52, 2665s.Sidransky, D., et al. 1992. Science256, 102.Hamer, P.J., et al. 1990. Hybridoma9, 573.Bos, J.L. 1989. Cancer Res.49, 4682.Furth, M. E., et al. 1987. Oncogene1, 47.Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA81, 5384.Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA80, 2112.Taparowsky, E., et al. 1983. Cell34, 581.Ellis, R.W., et al. 1981. Nature292, 506.Shih, T.Y., et al. 1980. Nature287, 686.

The level of expression of p21^ras is variable in different tissues. For this reason we recommend a concentration step prior to immunoblot analysis to obtain optimal results. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)